Developmental potential of Frankia vesicles.
نویسندگان
چکیده
The ability of nitrogenase-containing Frankia sp. strain CpI1 vesicles to regrow vegetative hyphae is demonstrated. Vesicles attached to hyphae in N2-fixing CpI1 cultures and sucrose gradient-isolated vesicles exhibited hyphal outgrowths when incubated in certain defined liquid media. Single or multiple hyphal extensions grew out from the vesicles.
منابع مشابه
Enzymes of ammonia assimilation in hyphae and vesicles of Frankia sp. strain CpI1.
Frankia spp. are filamentous actinomycetes that fix N2 in culture and in actinorhizal root nodules. In combined nitrogen-depleted aerobic environments, nitrogenase is restricted to thick-walled spherical structures, Frankia vesicles, that are formed on short stalks along the vegetative hyphae. The activities of the NH4(+)-assimilating enzymes (glutamine synthetase [GS], glutamate synthase, glut...
متن کاملIsolation and structure of the lipid envelopes from the nitrogen-fixing vesicles of Frankia sp. strain CpI1.
Frankia vesicles are differentiated during nitrogen starvation; they contain nitrogenase whether produced by free-living frankiae or by frankiae in actinorhizal root nodules. Vesicles are surrounded by envelopes of several monolayers of uncharacterized lipid. It has been suggested that the envelope limits diffusion of O2 into the vesicle cytoplasm, thereby preventing inactivation of nitrogenase...
متن کاملDifferences in fatty acid composition between vegetative cells and N(2)-fixing vesicles of Frankia sp. strain CpI1.
When growing on N(2), actinomycetes from the genus Frankia form multicellular structures that contain nitrogenase. The structures are referred to as vesicles and are indistinguishable from vesicles formed when Frankia sp. are in root-nodule symbioses. Vesicles isolated from N(2)-grown cells of Frankia sp. strain CpI1 had a significantly higher amount and different composition of fatty acids tha...
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A strain of Frankia, designated Cjl-82, from root nodules of a hybrid of Casuarina junghuhniana and C . equisetifolia has been isolated in pure culture. When grown under standard culture conditions, Cj 1-82 exhibited the following characteristics: cushionlike colonies with short, wide hyphae and, in addition to typical sporangia, intercalary elongated sporangialike structures (SLS) which could ...
متن کاملRoot nodule initiation in Gymnostoma (Casuarinaceae) and Shepherdia (Elaeagnaceae) induced by Frankia strain HFPGpI1
RACETTE, S., and TORREY, J. G. 1989. Root nodule initiation in Gymnostoma (Casuarinaceae) and Shepherdia (Elaeagnaceae) induced by Frankia strain HFPGpIl. Can. J. Bot. 67: 2873-2879. Seedlings of Gymnostoma papuanum (Casuarinaceae) and Shepherdia argentea (Elaeagnaceae) were grown in water culture and inoculated with Frankia strain HFPGpIl. Root nodule initiation and early nodule development we...
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عنوان ژورنال:
- Journal of bacteriology
دوره 171 12 شماره
صفحات -
تاریخ انتشار 1989